AD
Data & Repro
Adaptyv
Adaptyv is a cloud laboratory platform that provides automated protein testing and validation services. Submit protein sequences via API or…
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Data & Repro
BI
binding-characterization
Maintainer FreedomIntelligence · Last updated April 1, 2026
Guidance for SPR and BLI binding characterization experiments. Use when: (1) Planning binding kinetics experiments, (2) Troubleshooting poor/no binding signal, (3) Interpreting kinetic data artifacts, (4) Choosing between SPR vs BLI platforms.
Original source
FreedomIntelligence/OpenClaw-Medical-Skills
https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/binding-characterization
- Maintainer
- FreedomIntelligence
- License
- MIT
- Last updated
- April 1, 2026
Skill Snapshot
Key Details From SKILL.md
Source Doc
Excerpt From SKILL.md
SPR (Surface Plasmon Resonance)
- Mechanism: Detects refractive index changes at gold surface
- Surface: Gold chip with dextran matrix (CM5, CM7, etc.)
- Flow: Continuous microfluidics
- Best for: Small molecules, high-affinity, precise kon/koff
BLI (Biolayer Interferometry)
- Mechanism: Measures optical interference pattern shift
- Surface: Fiber optic biosensor tips (SA, Ni-NTA, AHC)
- Flow: Dip-and-read (no microfluidics)
- Best for: High-throughput, crude samples, antibody screening
Why SPR works but BLI doesn't
| Cause | Mechanism | Solution |
|---|---|---|
| Small analyte | BLI less sensitive for <10 kDa | Use SPR with appropriate chip |
| Weak affinity (KD >10μM) | Fast dissociation in BLI dip | Increase analyte concentration |
| Low expression | Not enough signal | Increase biosensor loading |
Use cases
- Use when: (1) Planning binding kinetics experiments, (2) Troubleshooting poor/no binding signal, (3) Interpreting kinetic data artifacts, (4) Choosing between SPR vs BLI platforms.
Not for
- Do not rely on this catalog entry alone for installation or maintenance details.
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