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bio-methylation-based-detection
Maintainer FreedomIntelligence · Last updated April 1, 2026
Analyzes cfDNA methylation patterns for cancer detection using cfMeDIP-seq or bisulfite sequencing with MethylDackel. Identifies cancer-specific methylation signatures and performs tissue-of-origin deconvolution. Use when using methylation biomarkers for early cancer detection or minimal residual disease.
Original source
FreedomIntelligence/OpenClaw-Medical-Skills
https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/bio-methylation-based-detection
- Maintainer
- FreedomIntelligence
- License
- MIT
- Last updated
- April 1, 2026
Skill Snapshot
Key Details From SKILL.md
Key Notes
- CLI: MethylDackel extract for methylation calling from cfDNA bisulfite data.
- Detect cancer from cfDNA methylation patterns" → Analyze cell-free DNA methylation for multi-cancer early detection and tissue-of-origin classification using bisulfite or enzymatic conversion. CLI: MethylDackel extract for methylation calling from cfDNA bisulfite data.
- Analyze cfDNA methylation for cancer detection and tissue-of-origin analysis.
- MethylDackel extract \ reference.fa \ sample_bismark.bam \ --CHG \ --CHH \ -o sample_methylation.
Source Doc
Excerpt From SKILL.md
Methods Overview
| Method | Description | cfDNA Input |
|---|---|---|
| cfMeDIP-seq | Enrichment-based, good for low input | >= 5 ng |
| Bisulfite-seq | Single-base resolution | >= 10 ng |
| EM-seq | Enzymatic, less degradation | >= 10 ng |
MethylDackel Pipeline
MethylDackel is actively maintained and integrated with nf-core/methylseq.
## Merge C and G strand calls
MethylDackel mergeContext \
reference.fa \
sample_methylation
Use cases
- Use when using methylation biomarkers for early cancer detection or minimal residual disease.
Not for
- Do not rely on this catalog entry alone for installation or maintenance details.
Upstream Related Skills
- cfdna-preprocessing - Preprocess before methylation analysis
- fragment-analysis - Complement with fragmentomics
- methylation-analysis/bismark-alignment - General methylation processing
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