bio-primer-design-primer-basics

Reverse complement for designing reverse primers

seq = 'ATGCGATCGATC' rc_seq = p3helpers.reverse_complement(seq) print(f'Reverse complement: {rc_seq}') # 'GATCGATCGCAT'

Ensure valid DNA sequence (ACGT only, uppercase)

valid_seq = p3helpers.ensure_acgt_uppercase('atgcNNgatc') # Raises error if invalid

## Target a specific region: [start, length]

result = primer3.design_primers(
    seq_args={
        'SEQUENCE_TEMPLATE': sequence,
        'SEQUENCE_TARGET': [100, 50],  # Target region at position 100, length 50
    },
    global_args={
        'PRIMER_PRODUCT_SIZE_RANGE': [[150, 300]],
        'PRIMER_OPT_TM': 60.0,
    }
)