Data & ReproProtein Structure & DesignFreedomIntelligence/OpenClaw-Medical-SkillsData & Reproduction
BI

bio-restriction-enzyme-selection

Maintainer FreedomIntelligence · Last updated April 1, 2026

Select restriction enzymes for cloning based on cut sites and compatibility.

OpenClawNanoClawAnalysisReproductionbio-restriction-enzyme-selection🧠 bioos extended suitebioos extended bioinformatics suiteselect

Original source

FreedomIntelligence/OpenClaw-Medical-Skills

https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/bio-restriction-enzyme-selection

Maintainer
FreedomIntelligence
License
MIT
Last updated
April 1, 2026

Skill Snapshot

Key Details From SKILL.md

2 min

Key Notes

  • once_cutters = analysis.once_cutters().
  • twice_cutters = analysis.twice_cutters().

Source Doc

Excerpt From SKILL.md

Find Enzymes by Cut Frequency

from Bio import SeqIO
from Bio.Restriction import Analysis, CommOnly, AllEnzymes

record = SeqIO.read('sequence.fasta', 'fasta')
seq = record.seq

analysis = Analysis(CommOnly, seq)

## Enzymes that don't cut (good for cloning insert)

non_cutters = analysis.only_dont_cut()

## All enzymes that cut (any number of times)

all_cutters = analysis.only_cut()

Use cases

  • Use bio-restriction-enzyme-selection in research workflows aligned with this subject area.
  • Follow the upstream documentation for the full working procedure.

Not for

  • Do not rely on this catalog entry alone for installation or maintenance details.

Upstream Related Skills

  • restriction-sites - Find where selected enzymes cut
  • restriction-mapping - Map selected enzyme sites
  • fragment-analysis - Predict fragments from selected enzymes

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