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bio-small-rna-seq-smrna-preprocessing

Maintainer FreedomIntelligence · Last updated April 1, 2026

Preprocess small RNA-seq: adapter trimming, size selection.

OpenClawNanoClawAnalysisReproductionbio-small-rna-seq-smrna-preprocessing🧠 bioos extended suitebioos extended bioinformatics suitepreprocess

Original source

FreedomIntelligence/OpenClaw-Medical-Skills

https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/bio-small-rna-seq-smrna-preprocessing

Maintainer: FreedomIntelligence
License: MIT
Last updated: April 1, 2026

Skill Snapshot

Key Details From SKILL.md

2 min

Key Notes

cutadapt \ -a TGGAATTCTCGGGTGCCAAGG \ -m 18 \ -M 30 \ --discard-untrimmed \ -o trimmed.fastq.gz \ input.fastq.gz.
cutadapt \ -a TGGAATTCTCGGGTGCCAAGG \ -m 18 -M 26 \ -o mirna_length.fastq.gz \ input.fastq.gz.

Source Doc

Excerpt From SKILL.md

Adapter Trimming with Cutadapt

Small RNA libraries have specific 3' adapters that must be removed:


## Common Small RNA Adapters

| Kit | 3' Adapter Sequence |
|-----|---------------------|
| Illumina TruSeq | TGGAATTCTCGGGTGCCAAGG |
| NEBNext | AGATCGGAAGAGCACACGTCT |
| QIAseq | AACTGTAGGCACCATCAAT |
| Lexogen | TGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC |

## Trim low-quality bases from 3' end before adapter removal

cutadapt \
    -q 20 \
    -a TGGAATTCTCGGGTGCCAAGG \
    -m 18 \
    -o trimmed.fastq.gz \
    input.fastq.gz

Use cases

Use bio-small-rna-seq-smrna-preprocessing for genomics and bioinformatics workflows.
Apply bio-small-rna-seq-smrna-preprocessing to sequencing, variant, or omics analysis tasks.