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Maintainer FreedomIntelligence · Last updated April 1, 2026
Metagenomics: QC → classification → functional profiling → AMR.
Original source
https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/bio-workflows-metagenomics-pipeline
Skill Snapshot
Source Doc
for sample in sample1 sample2 sample3; do
bowtie2 -p 8 -x human_index
-1 trimmed/${sample}_R1.fq.gz
-2 trimmed/${sample}_R2.fq.gz
--un-conc-gz host_removed/${sample}_R%.fq.gz
> /dev/null 2> qc/${sample}_host_removal.log
done
## Classify reads
for sample in sample1 sample2 sample3; do
kraken2 --db kraken2_db \
--threads 8 \
--paired \
--report kraken/${sample}.report \
--output kraken/${sample}.output \
host_removed/${sample}_R1.fq.gz \
host_removed/${sample}_R2.fq.gz
done
for sample in sample1 sample2 sample3; do
bracken -d kraken2_db
-i kraken/${sample}.report
-o bracken/${sample}.species.txt
-r 150
-l S
-t 10
done
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