AA
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aav-vector-design-agent
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Data & Repro
CR
crispr-guide-design
Maintainer FreedomIntelligence · Last updated April 1, 2026
CRISPR guide RNA design with on-target scoring and off-target minimization.
Original source
FreedomIntelligence/OpenClaw-Medical-Skills
https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/crispr-guide-design
- Maintainer
- FreedomIntelligence
- License
- MIT
- Last updated
- April 1, 2026
Skill Snapshot
Key Details From SKILL.md
Key Notes
- Automate sgRNA selection, scoring, off-target evaluation, and oligo generation for CRISPR experiments using the documented workflow.
- Designing CRISPR knockout/knock-in experiments that need validated guides.
- Locating all PAM-compatible target sites in a gene or locus.
- Filtering guides by efficiency/off-target metrics before cloning.
- Designing CRISPR knockout/knock-in experiments that need validated guides. Locating all PAM-compatible target sites in a gene or locus. Filtering guides by efficiency/off-target metrics before cloning.
Source Doc
Excerpt From SKILL.md
Core Capabilities
- Target discovery: Scan sequences for PAM motifs (e.g., NGG).
- Efficiency scoring: Evaluate GC content, homopolymers, Doench/DeepCRISPR/CFD scores.
- Filtering & ranking: Remove risky guides (SNP overlap, off-target hits) and output the best candidates.
Workflow
- Resolve gene symbol + organism to canonical transcript coordinates and target region.
- Enumerate PAM-compatible sites; extract spacers for the chosen Cas variant.
- Score guides (efficiency + specificity) and compute GC metrics.
- Run off-target search (≤3 mismatches) to flag problematic loci.
- Filter/rank guides, generate cloning oligos/primers, and emit JSON/CSV outputs with coordinates.
Guardrails
- Always state genome build and Cas variant assumptions.
- Avoid guides overlapping common SNPs when
avoid_variantsis true. - Flag high off-target density near coding regions for manual review.
Use cases
- Use crispr-guide-design for medicinal chemistry and drug-discovery work.
- Apply crispr-guide-design to compound, target, or screening workflows.
Not for
- Do not rely on this catalog entry alone for installation or maintenance details.
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