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tumor-heterogeneity-agent
Maintainer FreedomIntelligence · Last updated April 1, 2026
Analyze intratumoral heterogeneity from bulk and single-cell sequencing data.
Original source
FreedomIntelligence/OpenClaw-Medical-Skills
https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/tumor-heterogeneity-agent
- Maintainer
- FreedomIntelligence
- License
- MIT
- Last updated
- April 1, 2026
Skill Snapshot
Key Details From SKILL.md
Key Notes
- The Tumor Heterogeneity Agent provides comprehensive analysis of intratumor heterogeneity (ITH) for understanding clonal architecture, tracking subclonal evolution, and predicting therapy resistance. It integrates multi-region sequencing, single-cell data, and longitudinal samples to reconstruct tumor phylogenies and identify actionable subclones.
- When analyzing multi-region tumor sequencing for clonal architecture.
- For tracking clonal evolution under treatment pressure.
- To predict resistance emergence from subclonal populations.
- When assessing tumor heterogeneity impact on treatment response.
Source Doc
Excerpt From SKILL.md
Core Capabilities
-
Clonal Deconvolution: Infer clonal populations and their frequencies.
-
Phylogeny Reconstruction: Build tumor evolutionary trees from variants.
-
Subclonal Tracking: Monitor subclone dynamics over time.
-
Resistance Prediction: Identify pre-existing resistant subclones.
-
Multi-Region Integration: Combine spatial heterogeneity data.
-
Single-Cell ITH: Integrate scDNA-seq for ground-truth clones.
Heterogeneity Metrics
| Metric | Definition | Clinical Relevance |
|---|---|---|
| MATH Score | Mutant-allele tumor heterogeneity | ITH quantification |
| Shannon Index | Clonal diversity | Evolutionary potential |
| Clone Count | Number of distinct clones | Complexity |
| Truncal Fraction | % truncal mutations | Targetability |
| ITH Score | Composite heterogeneity | Prognosis |
Workflow
-
Input: Multi-region/longitudinal WES/WGS, copy number, tumor purity.
-
Preprocessing: Variant calling, CNV calling, purity estimation.
-
CCF Estimation: Calculate cancer cell fraction for each mutation.
-
Clustering: Group mutations into clonal populations.
-
Phylogeny: Reconstruct evolutionary tree.
-
Temporal Analysis: Track clone dynamics over time.
-
Output: Clone structures, phylogenies, heterogeneity metrics.
Use cases
- Use tumor-heterogeneity-agent for single-cell or spatial omics analysis.
- Apply tumor-heterogeneity-agent to clustering, integration, or trajectory workflows.
Not for
- Do not rely on this catalog entry alone for installation or maintenance details.
Upstream Related Skills
- ctDNA_Dynamics_MRD_Agent - Liquid biopsy tracking
- Single_Cell_CNV_Agent - scDNA-seq analysis
- HRD_Analysis_Agent - Genomic instability
- Pan_Cancer_MultiOmics_Agent - Multi-omic integration
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