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数据与复现
BI
bio-alignment-validation
维护者 FreedomIntelligence · 最近更新 2026年4月1日
Validate alignment file integrity and detect truncated/corrupt files.
原始来源
FreedomIntelligence/OpenClaw-Medical-Skills
https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/bio-alignment-validation
- 维护者
- FreedomIntelligence
- 许可
- MIT
- 最近更新
- 2026年4月1日
技能摘要
来自 SKILL.md 的关键信息
核心说明
- Post-alignment quality control to verify alignment quality 、 identify issues。
- Insert size should match 库 preparation protocol。
原始文档
SKILL.md 摘录
Expected Insert Sizes
| Library Type | Expected Size |
|---|---|
| Standard WGS | 300-500 bp |
| PCR-free | 350-550 bp |
| RNA-seq | 150-300 bp |
| ChIP-seq | 150-300 bp |
| ATAC-seq | Multimodal |
Proper Pairing Rate
Percentage of reads correctly paired.
Expected Rates
| Metric | Good | Marginal | Poor |
|---|---|---|---|
| Proper pair | > 90% | 80-90% | < 80% |
| Mapped | > 95% | 90-95% | < 90% |
| Singletons | < 5% | 5-10% | > 10% |
适用场景
- Use bio-alignment-validation ，用于 genomics 、 bioinformatics workflows。
- Apply bio-alignment-validation to sequencing，variant，或 omics analysis tasks。
不适用场景
- Do not rely on this catalog entry alone ，用于 installation 或 maintenance details。
上游相关技能
- bam-statistics - Basic flagstat and depth
- duplicate-handling - Mark/remove duplicates
- alignment-filtering - Filter low-quality reads
- chip-seq/chipseq-qc - ChIP-specific metrics
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