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维护者 FreedomIntelligence · 最近更新 2026年4月1日
Call RBP binding peaks from CLIP-seq with PureCLIP or MACS2.
原始来源
https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/bio-clip-seq-clip-peak-calling
技能摘要
原始文档
clipper
-b deduped.bam
-s hg38
--gene genes.bed
-o peaks.bed
## CLIPper Options
| Option | Description |
|--------|-------------|
| -b | Input BAM file |
| -s | Species (hg38, mm10) |
| -o | Output BED file |
| --FDR | FDR threshold (default 0.05) |
| --superlocal | Use superlocal background |
| --gene | Custom gene annotation BED |
| --save-pickle | Save intermediate data |
## PureCLIP (HMM-Based)
PureCLIP uses an HMM to model crosslink sites, incorporating enrichment and truncation signals.
```bash
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