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bio-crispr-screens-library-design
维护者 FreedomIntelligence · 最近更新 2026年4月1日
bio-crispr-screens-library-design：CRISPR 库 design ，用于 genetic screens。 Covers sgRNA selection，库 composition，control design，、 oligo ordering。 适合在designing custom sgRNA libraries ，用于 knockout，activation，或 interference screens时使用。
原始来源
FreedomIntelligence/OpenClaw-Medical-Skills
https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/bio-crispr-screens-library-design
- 维护者
- FreedomIntelligence
- 许可
- MIT
- 最近更新
- 2026年4月1日
技能摘要
来自 SKILL.md 的关键信息
核心说明
- Python：CRISPOR-based scoring ，支持 BioPython ，用于 sequence handling。
- Design custom CRISPR 库 ，用于 my screen" → Select optimal sgRNAs ，用于 knockout，CRISPRi/，或 base editing libraries ，支持 on-target scoring，off-target filtering，、 control guide design. Python：CRISPOR-based scoring ，支持 BioPython ，用于 sequence handling。
- def design_cas12a_guides(gene_sequence，n_guides=4)：pam_pattern = 'TTT[ACG]' # TTTV guide_length = 23。
- 用于 match in re.finditer(f'({pam_pattern})([ACGT]{{{guide_length}}})'，gene_sequence)：pam = match.group(1) guide = match.group(2) # Cas12a cuts downstream of guide #。
原始文档
SKILL.md 摘录
sgRNA Selection Criteria
Goal: Score and rank candidate sgRNAs for a target gene based on design quality metrics.
Approach: Scan the gene sequence for PAM sites, extract 20-nt protospacer sequences, score each on GC content, poly-T avoidance, 5' G preference, and length, then return the top-ranked candidates.
Library Composition
Goal: Assemble a complete sgRNA library targeting a list of genes with appropriate controls.
Approach: Design top-scoring guides for each gene, append non-targeting, essential-control, and safe-harbor-control guides, and compile into an ordered library table.
Control Guide Design
Goal: Design control guide sets for normalization and quality assessment in CRISPR screens.
Approach: Generate random non-targeting sequences with acceptable GC content, add validated guides against known essential genes (positive controls) and safe-harbor loci (negative controls).
适用场景
- 适合在designing custom sgRNA libraries ，用于 knockout，activation，或 interference screens时使用。
不适用场景
- Do not rely on this catalog entry alone ，用于 installation 或 maintenance details。
上游相关技能
- mageck-analysis - Analyze screen results
- crispresso-editing - Validate editing efficiency
- screen-qc - QC sequencing data
- hit-calling - Identify screen hits
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