AG
数据与复现
agent-browser
Browse the web for any task — research topics, read articles, interact with web apps, fill forms, take screenshots, extr…
FreedomIntelligence/OpenClaw-Medical-Skills查看
数据与复现
BI
bio-duplicate-handling
维护者 FreedomIntelligence · 最近更新 2026年4月1日
Mark and remove PCR duplicates with Picard or samtools markdup.
原始来源
FreedomIntelligence/OpenClaw-Medical-Skills
https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/bio-duplicate-handling
- 维护者
- FreedomIntelligence
- 许可
- MIT
- 最近更新
- 2026年4月1日
技能摘要
来自 SKILL.md 的关键信息
核心说明
- Mark 、 remove PCR/optical duplicates ，使用 samtools。
- samtools sort -n -o namesort.bam input.bam。
原始文档
SKILL.md 摘录
Why Remove Duplicates?
PCR duplicates are identical copies of the same original molecule, created during library preparation. They:
- Inflate coverage artificially
- Bias allele frequencies
- Can create false positive variant calls
Optical duplicates are clusters read multiple times due to their proximity on the flowcell.
Duplicate Marking Workflow
The standard samtools workflow requires multiple steps:
## 2. Add mate information with fixmate
samtools fixmate -m namesort.bam fixmate.bam
适用场景
- Use bio-duplicate-handling ，用于 genomics 、 bioinformatics workflows。
- Apply bio-duplicate-handling to sequencing，variant，或 omics analysis tasks。
不适用场景
- Do not rely on this catalog entry alone ，用于 installation 或 maintenance details。
上游相关技能
- alignment-sorting - Sort by name/coordinate for workflow
- alignment-filtering - Filter duplicates from output
- bam-statistics - Check duplicate rates with flagstat
- variant-calling - Duplicate marking before calling
相关技能
相关技能
AL
数据与复现
alpha-vantage
Access 20+ years of global financial data: equities, options, forex, crypto, commodities, economic indicators, and 50+ t…
K-Dense-AI/claude-scientific-skills查看
BI
Filter alignments by flag, quality, region, or paired status.
FreedomIntelligence/OpenClaw-Medical-Skills查看
BI
Index BAM/CRAM files with samtools index for random access.
FreedomIntelligence/OpenClaw-Medical-Skills查看