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bio-genome-assembly-assembly-polishing

维护者 FreedomIntelligence · 最近更新 2026年4月1日

Polish assemblies with Medaka (ONT) or NextPolish (Illumina).

OpenClawNanoClaw分析处理复现实验bio-genome-assembly-assembly-polishing🧠 bioos extended suitebioos extended bioinformatics suitepolish

原始来源

FreedomIntelligence/OpenClaw-Medical-Skills

https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/bio-genome-assembly-assembly-polishing

维护者
FreedomIntelligence
许可
MIT
最近更新
2026年4月1日

技能摘要

来自 SKILL.md 的关键信息

2 min

核心说明

  • Improve assembly accuracy by correcting errors ，使用 additional sequencing data。
  • bwa index assembly.fasta bwa mem -t 16 assembly.fasta R1.fq.gz R2.fq.gz | samtools sort -o aligned.bam samtools index aligned.bam。

原始文档

SKILL.md 摘录

Polishing Strategies

ToolInput ReadsBest For
PilonIlluminaFinal polishing
medakaONTONT assemblies
RaconLong readsQuick polishing
NextPolishBothCombined approach

ONT Assembly

  1. Racon (2-3 rounds with ONT)
  2. medaka (1 round)
  3. Pilon (2-3 rounds with Illumina)

PacBio CLR Assembly

  1. Racon (2-3 rounds)
  2. Pilon (2-3 rounds with Illumina)

适用场景

  • Use bio-genome-assembly-assembly-polishing ，用于 genomics 、 bioinformatics workflows。
  • Apply bio-genome-assembly-assembly-polishing to sequencing，variant，或 omics analysis tasks。

不适用场景

  • Do not rely on this catalog entry alone ，用于 installation 或 maintenance details。

上游相关技能

  • long-read-assembly - Initial assembly
  • short-read-assembly - Source of polishing reads
  • assembly-qc - Assess polishing improvement
  • long-read-sequencing - medaka variant calling

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