AG
数据与复现
agent-browser
Browse the web for any task — research topics, read articles, interact with web apps, fill forms, take screenshots, extr…
FreedomIntelligence/OpenClaw-Medical-Skills查看
数据与复现
BI
bio-long-read-sequencing-isoseq-analysis
维护者 FreedomIntelligence · 最近更新 2026年4月1日
Analyze PacBio Iso-Seq data for full-length isoform discovery and quantification. Use when characterizing transcript diversity or identifying novel splice variants.
原始来源
FreedomIntelligence/OpenClaw-Medical-Skills
https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/bio-long-read-sequencing-isoseq-analysis
- 维护者
- FreedomIntelligence
- 许可
- MIT
- 最近更新
- 2026年4月1日
技能摘要
来自 SKILL.md 的关键信息
核心说明
- CLI：isoseq3 refine → isoseq3 cluster → pbmm2 align → sqanti3_qc.py。
- 分析 full-length isoforms ，面向 my Iso-Seq data" → Process PacBio HiFi reads through CCS generation，primer removal，聚类，、 isoform 分类 to discover novel transcript variants. CLI：isoseq3 refine → isoseq3 cluster → pbmm2 align → sqanti3_qc.py。
- ccs input.subreads.bam ccs.bam \ --min-rq 0.9 \ --min-passes 3 \ --num-threads 32。
原始文档
SKILL.md 摘录
Iso-Seq specific primer removal
lima ccs.bam primers.fasta demux.bam
--isoseq
--peek-guess
--num-threads 16
Remove polyA tails and concatemers
isoseq3 refine demux.primer_5p--primer_3p.bam primers.fasta refined.bam
--require-polya
--min-polya-length 20
Check refinement stats
cat refined.filter_summary.json | jq
适用场景
- 适合在characterizing transcript diversity 或 identifying novel splice variants时使用。
不适用场景
- Do not rely on this catalog entry alone ，用于 installation 或 maintenance details。
上游相关技能
- long-read-sequencing/basecalling - ONT/PacBio basics
- rna-quantification/alignment-free-quant - Expression analysis
- genome-intervals/gtf-gff-handling - GTF/GFF handling
- differential-expression/timeseries-de - Differential isoform usage
相关技能
相关技能
AL
数据与复现
alpha-vantage
Access 20+ years of global financial data: equities, options, forex, crypto, commodities, economic indicators, and 50+ t…
K-Dense-AI/claude-scientific-skills查看
BI
Filter alignments by flag, quality, region, or paired status.
FreedomIntelligence/OpenClaw-Medical-Skills查看
BI
Index BAM/CRAM files with samtools index for random access.
FreedomIntelligence/OpenClaw-Medical-Skills查看