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维护者 FreedomIntelligence · 最近更新 2026年4月1日
QIIME2 command-line workflow for 16S/ITS amplicon analysis. Alternative to DADA2/phyloseq R workflow with built-in provenance tracking. Use when preferring CLI over R, needing reproducible provenance, or working within QIIME2 ecosystem.
原始来源
https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/bio-microbiome-qiime2-workflow
技能摘要
原始文档
qiime demux summarize
--i-data demux.qza
--o-visualization demux.qzv
## DADA2 denoising (creates ASV table + representative sequences)
qiime dada2 denoise-paired \
--i-demultiplexed-seqs demux.qza \
--p-trunc-len-f 240 \
--p-trunc-len-r 160 \
--p-trim-left-f 0 \
--p-trim-left-r 0 \
--p-max-ee-f 2 \
--p-max-ee-r 2 \
--p-n-threads 0 \
--o-table table.qza \
--o-representative-sequences rep-seqs.qza \
--o-denoising-stats denoising-stats.qza
## View denoising stats
qiime metadata tabulate \
--m-input-file denoising-stats.qza \
--o-visualization denoising-stats.qzv
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