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维护者 FreedomIntelligence · 最近更新 2026年4月1日
Design PCR primers with Primer3 for standard amplification.
原始来源
https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/bio-primer-design-primer-basics
技能摘要
原始文档
seq = 'ATGCGATCGATC' rc_seq = p3helpers.reverse_complement(seq) print(f'Reverse complement: {rc_seq}') # 'GATCGATCGCAT'
valid_seq = p3helpers.ensure_acgt_uppercase('atgcNNgatc') # Raises error if invalid
## Target a specific region: [start, length]
result = primer3.design_primers(
seq_args={
'SEQUENCE_TEMPLATE': sequence,
'SEQUENCE_TARGET': [100, 50], # Target region at position 100, length 50
},
global_args={
'PRIMER_PRODUCT_SIZE_RANGE': [[150, 300]],
'PRIMER_OPT_TM': 60.0,
}
)
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