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FreedomIntelligence/OpenClaw-Medical-Skills查看
维护者 FreedomIntelligence · 最近更新 2026年4月1日
Splice-aware RNA-seq alignment with HISAT2.
原始来源
https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/bio-read-alignment-hisat2-alignment
技能摘要
原始文档
hisat2 -p 8 -x hisat2_index
-1 reads_1.fq.gz -2 reads_2.fq.gz
-S aligned.sam
hisat2 -p 8 -x hisat2_index
-U reads.fq.gz
-S aligned.sam
## Pipe to samtools
hisat2 -p 8 -x hisat2_index \
-1 r1.fq.gz -2 r2.fq.gz | \
samtools sort -@ 4 -o aligned.sorted.bam -
samtools index aligned.sorted.bam
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