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维护者 FreedomIntelligence · 最近更新 2026年4月1日
High-speed STAR aligner for RNA-seq with junction detection.
原始来源
https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/bio-read-alignment-star-alignment
技能摘要
原始文档
STAR --runThreadN 8
--genomeDir star_index/
--readFilesIn reads_1.fq.gz reads_2.fq.gz
--readFilesCommand zcat
--outFileNamePrefix sample_
--outSAMtype BAM SortedByCoordinate
## Two-pass mode for better novel junction detection
STAR --runThreadN 8 \
--genomeDir star_index/ \
--readFilesIn r1.fq.gz r2.fq.gz \
--readFilesCommand zcat \
--outFileNamePrefix sample_ \
--outSAMtype BAM SortedByCoordinate \
--twopassMode Basic
STAR --runThreadN 8
--genomeDir star_index/
--readFilesIn r1.fq.gz r2.fq.gz
--readFilesCommand zcat
--outFileNamePrefix sample_
--outSAMtype BAM SortedByCoordinate
--quantMode GeneCounts
Output: `sample_ReadsPerGene.out.tab` with columns:
1. Gene ID
2. Unstranded counts
3. Forward strand counts
4. Reverse strand counts
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