数据与复现生物信息与基因组学FreedomIntelligence/OpenClaw-Medical-Skills数据与复现
BI

bio-read-alignment-star-alignment

维护者 FreedomIntelligence · 最近更新 2026年4月1日

High-speed STAR aligner for RNA-seq with junction detection.

OpenClawNanoClaw分析处理复现实验bio-read-alignment-star-alignment🧠 bioos extended suitebioos extended bioinformatics suitehigh

原始来源

FreedomIntelligence/OpenClaw-Medical-Skills

https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/bio-read-alignment-star-alignment

维护者
FreedomIntelligence
许可
MIT
最近更新
2026年4月1日

技能摘要

来自 SKILL.md 的关键信息

2 min

核心说明

  • STAR --runMode genomeGenerate \ --runThreadN 8 \ --genomeDir star_index/ \ --genomeFastaFiles reference.fa \ --sjdbGTFfile annotation.gtf \ --sjdbOverhang 100 # Read length - 1。
  • STAR --runMode genomeGenerate \ --runThreadN 8 \ --genomeDir star_index_150/ \ --genomeFastaFiles reference.fa \ --sjdbGTFfile annotation.gtf \ --sjdbOverhang 149。

原始文档

SKILL.md 摘录

Paired-end alignment

STAR --runThreadN 8
--genomeDir star_index/
--readFilesIn reads_1.fq.gz reads_2.fq.gz
--readFilesCommand zcat
--outFileNamePrefix sample_
--outSAMtype BAM SortedByCoordinate


## Two-pass mode for better novel junction detection

STAR --runThreadN 8 \
    --genomeDir star_index/ \
    --readFilesIn r1.fq.gz r2.fq.gz \
    --readFilesCommand zcat \
    --outFileNamePrefix sample_ \
    --outSAMtype BAM SortedByCoordinate \
    --twopassMode Basic

Output gene counts (like featureCounts)

STAR --runThreadN 8
--genomeDir star_index/
--readFilesIn r1.fq.gz r2.fq.gz
--readFilesCommand zcat
--outFileNamePrefix sample_
--outSAMtype BAM SortedByCoordinate
--quantMode GeneCounts


Output: `sample_ReadsPerGene.out.tab` with columns:
1. Gene ID
2. Unstranded counts
3. Forward strand counts
4. Reverse strand counts

适用场景

  • Use bio-read-alignment-star-alignment ,用于 genomics 、 bioinformatics workflows。
  • Apply bio-read-alignment-star-alignment to sequencing,variant,或 omics analysis tasks。

不适用场景

  • Do not rely on this catalog entry alone ,用于 installation 或 maintenance details。

上游相关技能

  • rna-quantification/featurecounts-counting - Alternative counting
  • rna-quantification/alignment-free-quant - Salmon/kallisto alternative
  • differential-expression/deseq2-basics - Downstream DE analysis
  • read-qc/fastp-workflow - Preprocess reads

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