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bio-restriction-enzyme-selection
维护者 FreedomIntelligence · 最近更新 2026年4月1日
Select restriction enzymes for cloning based on cut sites and compatibility.
原始来源
FreedomIntelligence/OpenClaw-Medical-Skills
https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/bio-restriction-enzyme-selection
- 维护者
- FreedomIntelligence
- 许可
- MIT
- 最近更新
- 2026年4月1日
技能摘要
来自 SKILL.md 的关键信息
核心说明
- once_cutters = analysis.once_cutters()。
- twice_cutters = analysis.twice_cutters()。
原始文档
SKILL.md 摘录
Find Enzymes by Cut Frequency
from Bio import SeqIO
from Bio.Restriction import Analysis, CommOnly, AllEnzymes
record = SeqIO.read('sequence.fasta', 'fasta')
seq = record.seq
analysis = Analysis(CommOnly, seq)
## Enzymes that don't cut (good for cloning insert)
non_cutters = analysis.only_dont_cut()
## All enzymes that cut (any number of times)
all_cutters = analysis.only_cut()
适用场景
- Use bio-restriction-enzyme-selection in 科研工作流 aligned ，支持 this subject area。
- Follow upstream documentation ，用于 full working procedure。
不适用场景
- Do not rely on this catalog entry alone ，用于 installation 或 maintenance details。
上游相关技能
- restriction-sites - Find where selected enzymes cut
- restriction-mapping - Map selected enzyme sites
- fragment-analysis - Predict fragments from selected enzymes
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