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维护者 FreedomIntelligence · 最近更新 2026年4月1日
Pseudo-alignment quantification with Salmon or Kallisto.
原始来源
https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/bio-rna-quantification-alignment-free-quant
技能摘要
原始文档
grep "^>" genome.fa | cut -d " " -f 1 | sed 's/>//g' > decoys.txt cat transcripts.fa genome.fa > gentrome.fa salmon index -t gentrome.fa -d decoys.txt -i salmon_index -p 8
## Paired-end reads
salmon quant -i salmon_index -l A \
-1 sample_R1.fastq.gz -2 sample_R2.fastq.gz \
-o sample_quant -p 8
## Single-end reads
salmon quant -i salmon_index -l A \
-r sample.fastq.gz \
-o sample_quant -p 8
Key flags:
-l A - Automatically detect library type-p - Number of threads--validateMappings - More accurate (default in recent versions)--gcBias - Correct for GC bias--seqBias - Correct for sequence-specific bias相关技能
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