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bio-rna-quantification-featurecounts-counting

维护者 FreedomIntelligence · 最近更新 2026年4月1日

Count reads per gene with featureCounts from subread package.

OpenClawNanoClaw分析处理复现实验bio-rna-quantification-featurecounts-counting🧠 bioos extended suitebioos extended bioinformatics suitecount

原始来源

FreedomIntelligence/OpenClaw-Medical-Skills

https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/bio-rna-quantification-featurecounts-counting

维护者
FreedomIntelligence
许可
MIT
最近更新
2026年4月1日

技能摘要

来自 SKILL.md 的关键信息

2 min

核心说明

  • Count reads mapping to genomic features (genes，exons) ，面向 BAM files。
  • featureCounts - annotation.gtf -o counts.txt aligned.bam。

原始文档

SKILL.md 摘录

Multiple samples (recommended - single matrix output)

featureCounts -a annotation.gtf -o counts.txt sample1.bam sample2.bam sample3.bam

All BAMs in directory

featureCounts -a annotation.gtf -o counts.txt *.bam


## Count fragments, not reads (required for paired-end)

featureCounts -p --countReadPairs -a annotation.gtf -o counts.txt *.bam

适用场景

  • Use bio-rna-quantification-featurecounts-counting ，用于 genomics 、 bioinformatics workflows。
  • Apply bio-rna-quantification-featurecounts-counting to sequencing，variant，或 omics analysis tasks。

不适用场景

  • Do not rely on this catalog entry alone ，用于 installation 或 maintenance details。

上游相关技能

  • alignment-files/sam-bam-basics - Input BAM file handling
  • genome-intervals/gtf-gff-handling - GTF annotation files
  • differential-expression/deseq2-basics - Downstream analysis with counts
  • rna-quantification/count-matrix-qc - QC of count data

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