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bio-small-rna-seq-mirdeep2-analysis
维护者 FreedomIntelligence · 最近更新 2026年4月1日
Identify and quantify known/novel miRNAs with miRDeep2.
原始来源
FreedomIntelligence/OpenClaw-Medical-Skills
https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/bio-small-rna-seq-mirdeep2-analysis
- 维护者
- FreedomIntelligence
- 许可
- MIT
- 最近更新
- 2026年4月1日
技能摘要
来自 SKILL.md 的关键信息
核心说明
- bowtie-build genome.fa genome_index。
- mapper.pl reads.fastq \ -e \ -h \ -i \ -j \ -k TGGAATTCTCGGGTGCCAAGG \ -l 18 \ -m \ -p genome_index \ -s reads_collapsed.fa \ -t reads_vs_genome.arf \ -v。
原始文档
SKILL.md 摘录
Predict novel miRNAs
miRDeep2.pl
reads_collapsed.fa
genome.fa
reads_vs_genome.arf
mature_ref.fa
mature_other.fa
hairpin_ref.fa
-t Human
2> report.log
Download from miRBase
wget https://www.mirbase.org/download/mature.fa wget https://www.mirbase.org/download/hairpin.fa
Extract species-specific sequences
grep -A1 ">hsa-" mature.fa > mature_human.fa grep -A1 ">hsa-" hairpin.fa > hairpin_human.fa
适用场景
- Use bio-small-rna-seq-mirdeep2-analysis ，用于 genomics 、 bioinformatics workflows。
- Apply bio-small-rna-seq-mirdeep2-analysis to sequencing，variant，或 omics analysis tasks。
不适用场景
- Do not rely on this catalog entry alone ，用于 installation 或 maintenance details。
上游相关技能
- smrna-preprocessing - Prepare reads for miRDeep2
- mirge3-analysis - Faster quantification alternative
- differential-mirna - DE analysis of miRNA counts
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