数据与复现生物信息与基因组学FreedomIntelligence/OpenClaw-Medical-Skills数据与复现

bio-small-rna-seq-smrna-preprocessing

维护者 FreedomIntelligence · 最近更新 2026年4月1日

Preprocess small RNA-seq: adapter trimming, size selection.

OpenClawNanoClaw分析处理复现实验bio-small-rna-seq-smrna-preprocessing🧠 bioos extended suitebioos extended bioinformatics suitepreprocess

原始来源

FreedomIntelligence/OpenClaw-Medical-Skills

https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/bio-small-rna-seq-smrna-preprocessing

维护者: FreedomIntelligence
许可: MIT
最近更新: 2026年4月1日

技能摘要

来自 SKILL.md 的关键信息

2 min

核心说明

cutadapt \ - TGGAATTCTCGGGTGCCAAGG \ -m 18 \ -M 30 \ --discard-untrimmed \ -o trimmed.fastq.gz \ input.fastq.gz。
cutadapt \ - TGGAATTCTCGGGTGCCAAGG \ -m 18 -M 26 \ -o mirna_length.fastq.gz \ input.fastq.gz。

原始文档

SKILL.md 摘录

Adapter Trimming with Cutadapt

Small RNA libraries have specific 3' adapters that must be removed:


## Common Small RNA Adapters

| Kit | 3' Adapter Sequence |
|-----|---------------------|
| Illumina TruSeq | TGGAATTCTCGGGTGCCAAGG |
| NEBNext | AGATCGGAAGAGCACACGTCT |
| QIAseq | AACTGTAGGCACCATCAAT |
| Lexogen | TGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC |

## Trim low-quality bases from 3' end before adapter removal

cutadapt \
    -q 20 \
    -a TGGAATTCTCGGGTGCCAAGG \
    -m 18 \
    -o trimmed.fastq.gz \
    input.fastq.gz

适用场景

Use bio-small-rna-seq-smrna-preprocessing ，用于 genomics 、 bioinformatics workflows。
Apply bio-small-rna-seq-smrna-preprocessing to sequencing，variant，或 omics analysis tasks。