数据与复现生物信息与基因组学FreedomIntelligence/OpenClaw-Medical-Skills数据与复现
BI

bio-workflows-cytometry-pipeline

维护者 FreedomIntelligence · 最近更新 2026年4月1日

Flow/mass cytometry: QC → gating → clustering → differential.

OpenClawNanoClaw分析处理复现实验bio-workflows-cytometry-pipeline🧠 bioos extended suitebioos extended bioinformatics suiteflow

原始来源

FreedomIntelligence/OpenClaw-Medical-Skills

https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/bio-workflows-cytometry-pipeline

维护者
FreedomIntelligence
许可
MIT
最近更新
2026年4月1日

技能摘要

来自 SKILL.md 的关键信息

2 min

核心说明

  • panel <- data.frame( fcs_colname = c('FSC- ','SSC- ','CD45','CD3','CD4','CD8','CD19','CD14','CD56','HLA-DR','Ki67','IFNg'),antigen = c('FSC','SSC','CD45','CD3','CD4','CD8','CD19','CD14','CD56','HLA-DR','Ki67','IFNg'),marker_class = c('none','none','type','type','type','type','type','type','type','type','state','state') )。
  • md <- data.frame( file_name = list.files('data/',pattern = '\\.fcs$'),sample_id = paste0('Sample',1:8),condition = rep(c('Control','Treatment'),each = 4),patient_id = rep(paste0('Patient',1:4),2) )。
  • cat('Loading',nrow(md),'FCS files...\n')。

原始文档

SKILL.md 摘录

Complete R Workflow (CATALYST)

library(CATALYST)
library(diffcyt)
library(SingleCellExperiment)
library(flowCore)
library(ggplot2)

## === 2. LOAD AND PREPARE DATA ===

fcs_files <- file.path('data', md$file_name)
fs <- read.flowSet(fcs_files)

## Apply compensation if stored in FCS

fs_comp <- compensate(fs, spillover(fs[[1]]))

适用场景

  • Use bio-workflows-cytometry-pipeline ,用于 genomics 、 bioinformatics workflows。
  • Apply bio-workflows-cytometry-pipeline to sequencing,variant,或 omics analysis tasks。

不适用场景

  • Do not rely on this catalog entry alone ,用于 installation 或 maintenance details。

上游相关技能

  • flow-cytometry/fcs-handling - FCS file operations
  • flow-cytometry/compensation-transformation - Data preprocessing
  • flow-cytometry/gating-analysis - Manual gating
  • flow-cytometry/clustering-phenotyping - Unsupervised clustering
  • flow-cytometry/differential-analysis - Statistical testing
  • flow-cytometry/doublet-detection - Remove doublet events

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