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数据与复现
BI
bio-workflows-merip-pipeline
维护者 FreedomIntelligence · 最近更新 2026年4月1日
MeRIP-seq: m6A peak calling → differential → motif analysis.
原始来源
FreedomIntelligence/OpenClaw-Medical-Skills
https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/bio-workflows-merip-pipeline
- 维护者
- FreedomIntelligence
- 许可
- MIT
- 最近更新
- 2026年4月1日
技能摘要
来自 SKILL.md 的关键信息
原始文档
SKILL.md 摘录
QC Checkpoints
| Checkpoint | Expected | Action if Failed |
|---|---|---|
| IP/Input alignment rate | >80% | Check adapter contamination |
| IP/Input correlation | r < 0.8 | Verify IP enrichment |
| Peak count | 10,000-50,000 | Adjust -q threshold |
| DRACH motif in peaks | >50% | Check peak calling parameters |
| Stop codon enrichment | Clear peak | Confirm m6A signal |
Output Files
| File | Description |
|---|---|
m6a_peaks.bed | Called m6A peak locations |
m6a_peaks_annotated.txt | Peaks with gene annotations |
diff_m6a.csv | Differential methylation results |
metagene.pdf | Peak distribution across transcripts |
motif_output/ | Enriched motifs (expect DRACH) |
适用场景
- Use bio-workflows-merip-pipeline ，用于 genomics 、 bioinformatics workflows。
- Apply bio-workflows-merip-pipeline to sequencing，variant，或 omics analysis tasks。
不适用场景
- Do not rely on this catalog entry alone ，用于 installation 或 maintenance details。
上游相关技能
- epitranscriptomics/m6a-peak-calling - Detailed peak calling options
- epitranscriptomics/m6a-differential - Differential analysis methods
- epitranscriptomics/modification-visualization - Visualization techniques
- chip-seq/peak-calling - Similar IP-based peak calling concepts
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