数据与复现生物信息与基因组学FreedomIntelligence/OpenClaw-Medical-Skills数据与复现
BI

bio-workflows-methylation-pipeline

维护者 FreedomIntelligence · 最近更新 2026年4月1日

WGBS/RRBS: bismark alignment → methylation calling → DMR detection.

OpenClawNanoClaw分析处理复现实验bio-workflows-methylation-pipeline🧠 bioos extended suitebioos extended bioinformatics suitewgbs

原始来源

FreedomIntelligence/OpenClaw-Medical-Skills

https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/bio-workflows-methylation-pipeline

维护者
FreedomIntelligence
许可
MIT
最近更新
2026年4月1日

技能摘要

来自 SKILL.md 的关键信息

2 min

核心说明

  • Complete workflow ,面向 bisulfite sequencing FASTQ to differentially methylated regions。
  • trim_galore --paired --fastqc \ -o trimmed/ \ sample_R1.fastq.gz sample_R2.fastq.gz。

原始文档

SKILL.md 摘录

Or fastp with conservative settings

fastp -i sample_R1.fastq.gz -I sample_R2.fastq.gz
-o trimmed/sample_R1.fq.gz -O trimmed/sample_R2.fq.gz
--detect_adapter_for_pe
--qualified_quality_phred 20
--length_required 35
--html qc/sample_fastp.html


## Prepare genome (once)

bismark_genome_preparation --bowtie2 genome/

## Align

bismark --genome genome/ \
    -1 trimmed/sample_R1_val_1.fq.gz \
    -2 trimmed/sample_R2_val_2.fq.gz \
    -o aligned/ \
    --parallel 4 \
    --temp_dir tmp/

适用场景

  • Use bio-workflows-methylation-pipeline ,用于 genomics 、 bioinformatics workflows。
  • Apply bio-workflows-methylation-pipeline to sequencing,variant,或 omics analysis tasks。

不适用场景

  • Do not rely on this catalog entry alone ,用于 installation 或 maintenance details。

上游相关技能

  • methylation-analysis/bismark-alignment - Bismark parameters
  • methylation-analysis/methylation-calling - Calling details
  • methylation-analysis/methylkit-analysis - methylKit functions
  • methylation-analysis/dmr-detection - DMR algorithms

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