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维护者 FreedomIntelligence · 最近更新 2026年4月1日
WGBS/RRBS: bismark alignment → methylation calling → DMR detection.
原始来源
https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/bio-workflows-methylation-pipeline
技能摘要
原始文档
fastp -i sample_R1.fastq.gz -I sample_R2.fastq.gz
-o trimmed/sample_R1.fq.gz -O trimmed/sample_R2.fq.gz
--detect_adapter_for_pe
--qualified_quality_phred 20
--length_required 35
--html qc/sample_fastp.html
## Prepare genome (once)
bismark_genome_preparation --bowtie2 genome/
## Align
bismark --genome genome/ \
-1 trimmed/sample_R1_val_1.fq.gz \
-2 trimmed/sample_R2_val_2.fq.gz \
-o aligned/ \
--parallel 4 \
--temp_dir tmp/
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