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维护者 FreedomIntelligence · 最近更新 2026年4月1日
Ribo-seq: footprint alignment → periodicity → ORF detection.
原始来源
https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/bio-workflows-riboseq-pipeline
技能摘要
原始文档
STAR --genomeDir star_index
--readFilesIn non_rrna.fastq.gz
--readFilesCommand zcat
--outFilterMismatchNmax 2
--alignEndsType EndToEnd
--outSAMtype BAM SortedByCoordinate
## Step 3: P-site Calibration
```python
from plastid import BAMGenomeArray
## Build metagene profile
metagene_generate annotation.gtf ribo.bam metagene_output/
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