AA
AAV vector design: capsid selection, promoter optimization, payload capacity.
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数据与复现
CR
crispr-guide-design
维护者 FreedomIntelligence · 最近更新 2026年4月1日
CRISPR guide RNA design with on-target scoring and off-target minimization.
原始来源
FreedomIntelligence/OpenClaw-Medical-Skills
https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills/tree/main/skills/crispr-guide-design
- 维护者
- FreedomIntelligence
- 许可
- MIT
- 最近更新
- 2026年4月1日
技能摘要
来自 SKILL.md 的关键信息
核心说明
- Automate sgRNA selection，scoring，off-target evaluation，、 oligo generation ，用于 CRISPR experiments ，使用 documented workflow。
- Designing CRISPR knockout/knock-in experiments that need validated guides。
- Locating all PAM-compatible target sites in gene 或 locus。
- Filtering guides by efficiency/off-target metrics before cloning。
- Designing CRISPR knockout/knock-in experiments that need validated guides. Locating all PAM-compatible target sites in gene 或 locus. Filtering guides by efficiency/off-target metrics before cloning。
原始文档
SKILL.md 摘录
Core Capabilities
- Target discovery: Scan sequences for PAM motifs (e.g., NGG).
- Efficiency scoring: Evaluate GC content, homopolymers, Doench/DeepCRISPR/CFD scores.
- Filtering & ranking: Remove risky guides (SNP overlap, off-target hits) and output the best candidates.
Workflow
- Resolve gene symbol + organism to canonical transcript coordinates and target region.
- Enumerate PAM-compatible sites; extract spacers for the chosen Cas variant.
- Score guides (efficiency + specificity) and compute GC metrics.
- Run off-target search (≤3 mismatches) to flag problematic loci.
- Filter/rank guides, generate cloning oligos/primers, and emit JSON/CSV outputs with coordinates.
Guardrails
- Always state genome build and Cas variant assumptions.
- Avoid guides overlapping common SNPs when
avoid_variantsis true. - Flag high off-target density near coding regions for manual review.
适用场景
- Use crispr-guide-design ，用于 medicinal chemistry 、 drug-discovery work。
- Apply crispr-guide-design to compound，target，或 screening workflows。
不适用场景
- Do not rely on this catalog entry alone ，用于 installation 或 maintenance details。
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